Journal: Oxidative medicine and cellular longevity

Article Title: Continuous Infusion of Angiotensin IV Protects against Acute Myocardial Infarction via the Inhibition of Inflammation and Autophagy.

doi: 10.1155/2021/2860488

Figure Lengend Snippet: Figure 7: Ang IV reduced hypoxia-induced apoptosis and autophagy in NRVCs. (a, b) TUNEL staining showed that Ang IV reduced hypoxia-induced cardiomyocyte apoptosis. Bar = 100 μm. (c, d) Western blot analysis showed that Ang IV reduced the transformation of LC3-I to LC3-II but increased p62 expression. Ang: angiotensin; NRVCs: the neonatal rat ventricular myocytes. DAPI: blue; TUNEL: green; ∗P < 0:05 vs. the control group; #P < 0:05 vs. the hypoxia group.

Article Snippet: The primary antibodies used were as follows: rabbit antiMCP-1 antibody (Cell Signaling Technology, MA, USA), rat ICAM-1 antibody (1 : 500; R&D Systems, MN, USA), rabbit anti-iNOS antibody (1 : 1000; Cell Signaling Technology), rabbit anti-LC3 antibody (1 : 1000; Cell Signaling Technology), rabbit anti-P62 antibody (1 : 1000; Cell Signaling Technology), and rabbit anti-GAPDH antibody (1 : 1000; Cell Signaling Technology).

Techniques: TUNEL Assay, Staining, Western Blot, Transformation Assay, Expressing, Control

Journal: Oxidative medicine and cellular longevity

Article Title: Continuous Infusion of Angiotensin IV Protects against Acute Myocardial Infarction via the Inhibition of Inflammation and Autophagy.

doi: 10.1155/2021/2860488

Figure Lengend Snippet: Figure 7: Ang IV reduced hypoxia-induced apoptosis and autophagy in NRVCs. (a, b) TUNEL staining showed that Ang IV reduced hypoxia-induced cardiomyocyte apoptosis. Bar = 100 μm. (c, d) Western blot analysis showed that Ang IV reduced the transformation of LC3-I to LC3-II but increased p62 expression. Ang: angiotensin; NRVCs: the neonatal rat ventricular myocytes. DAPI: blue; TUNEL: green; ∗P < 0:05 vs. the control group; #P < 0:05 vs. the hypoxia group.

Article Snippet: The primary antibodies used were as follows: rabbit antiMCP-1 antibody (Cell Signaling Technology, MA, USA), rat ICAM-1 antibody (1 : 500; R&D Systems, MN, USA), rabbit anti-iNOS antibody (1 : 1000; Cell Signaling Technology), rabbit anti-LC3 antibody (1 : 1000; Cell Signaling Technology), rabbit anti-P62 antibody (1 : 1000; Cell Signaling Technology), and rabbit anti-GAPDH antibody (1 : 1000; Cell Signaling Technology).

Techniques: TUNEL Assay, Staining, Western Blot, Transformation Assay, Expressing, Control

Journal: Drug Design, Development and Therapy

Article Title:

Resveratrol downregulates TNF-α-induced monocyte chemoattractant protein-1 in primary rat pulmonary artery endothelial cells by P38 mitogen-activated protein kinase signaling

doi: 10.2147/dddt.s184785

Figure Lengend Snippet: Figure 7 Effect of resveratrol on protein expression of MCP-1 and p-p38MAPK in pulmonary artery cells. Pulmonary artery arterial cells were obtained from rats treated with resveratrol, and levels of MCP-1protein expression were measured by immunoblotting at 8 hrs time point. β-actin was used to normalize protein loading (mean ± SD, n=6 each group). ★p<0.05: RPAs-TNF-α vs RPAs-control, RPAs-1% DMSO, RPAs-TNF-α + resveratrol, RPAs-TNF-α + SB203580, RPAs-Resveratrol, RPAs-SB203580, and RPAs-TNF-α + resveratrol + SB203580. #p<0.05: RPAs-TNF-α + resveratrol and RPAs-TNF-α + SB203580 vs RPAs-TNF-α; ** p<0.05: RPAs-TNF-α + resveratrol + SB203580 vs RPAs-TNF-α + resveratrol and RPAs-TNF-α + SB203580. Abbreviation: RPAs, rat pulmonary artery endothelial cells.

Article Snippet: Immunoblotting After determination of total tissue protein concentration by BCA protein assay kit, 40 μg of the protein prepared above was separated on 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, then transferred the separated protein was to a PVDF membrane, and using 5% fat free milk-TBST buffer blocked the membrane for 2 hrs at room temperature; incubated it with the rabbit antibodies of antiMCP-1 (ab9669,1:500, Abcam, UK), anti-total p38MAPK (#8690, 1:1000, Cell Signaling Technology, USA), anti-pp38MAPK (9211S,1:1000, Cell Signaling Technology, USA) and anti β-actin antibody (A2228, 1: 2000, Sigma– Aldrich, USA) at 4°C overnight, followed with 1:5,000 secondary goat anti-rabbit antibodies incubation for 2 hrs (ab6721, Abcam, UK).

Techniques: Expressing, Western Blot, Control

Journal: Drug Design, Development and Therapy

Article Title:

Resveratrol downregulates TNF-α-induced monocyte chemoattractant protein-1 in primary rat pulmonary artery endothelial cells by P38 mitogen-activated protein kinase signaling

doi: 10.2147/dddt.s184785

Figure Lengend Snippet: Figure 8 MCP-1 mRNA expression mediated by the p38 MAPK-dependent signaling pathway in pulmonary artery cells. The levels of MCP-1 mRNA expression were measured using real-time PCR assay at 8 hrs time point in pulmonary artery cells (mean ± SD, n=6 each group). ★p<0.05: RPAs-TNF-α vs RPAs-control, RPAs-TNF-α + resveratrol, RPAs-TNF-α + SB203580 and RPAs-TNF-α + resveratrol + SB203580groups. #p<0.05: RPAs-TNF-α + resveratrol and RPAs-TNF-α + SB203580 vs RPAs-control and RPAs-TNF-α + resveratrol + SB203580; **p<0.05: RPAs-TNF-α + resveratrol + SB203580 vs RPAs-control. Abbreviation: RPAs, rat pulmonary artery endothelial cells.

Article Snippet: Immunoblotting After determination of total tissue protein concentration by BCA protein assay kit, 40 μg of the protein prepared above was separated on 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, then transferred the separated protein was to a PVDF membrane, and using 5% fat free milk-TBST buffer blocked the membrane for 2 hrs at room temperature; incubated it with the rabbit antibodies of antiMCP-1 (ab9669,1:500, Abcam, UK), anti-total p38MAPK (#8690, 1:1000, Cell Signaling Technology, USA), anti-pp38MAPK (9211S,1:1000, Cell Signaling Technology, USA) and anti β-actin antibody (A2228, 1: 2000, Sigma– Aldrich, USA) at 4°C overnight, followed with 1:5,000 secondary goat anti-rabbit antibodies incubation for 2 hrs (ab6721, Abcam, UK).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control